Development of <i>α</i>-Amino Acid Detection with Peroxyoxalate Chemiluminescence by Using Water-Acetonitrile-Ethyl Acetate Mixed Solution
نویسندگان
چکیده
منابع مشابه
Development of an immune microanalysis system by use of peroxyoxalate chemiluminescence detection.
We developed an immune microanalysis system incorporating chemiluminescence detection, where the peroxyoxalate chemiluminescence (CL) detection using bis[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)phenyl]oxalate (TDPO)-hydrogen peroxide (H2O2)-fluorescein isothiocianate (FITC) reaction was newly adopted. The analysis system performed the following three processes on a microchip: immune reaction fo...
متن کاملHPLC determination of methylphenidate and its metabolite, ritalinic acid, by high-performance liquid chromatography with peroxyoxalate chemiluminescence detection.
An HPLC-peroxyoxalate chemiluminescence (PO-CL) method for simultaneous determination of methylphenidate (MPH) and ritalinic acid (RA) was developed. The method was used to monitor MPH and RA after administration of MPH to rats. Deproteinized plasma spiked with 1-(3-trifluoromethylphenyl)piperazine (IS) was dried and labeled with 4-(N,N-dimethylaminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole (DBD-...
متن کاملDetermination of C-21 ketosteroids in serum using trifluoromethanesulfonic acid catalyzed precolumn dansylation and 1,1'-oxalyldiimidazole postcolumn peroxyoxalate chemiluminescence detection.
A new procedure for the quantitation of C-21 ketosteroids using trifluoromethanesulfonic acid-catalyzed precolumn dansylation and coupled column liquid chromatographic separation, followed by postcolumn 1,1'-oxalyldiimidazole peroxyoxalate chemiluminescence detection is presented. In the simultaneous optimization of chromatographic resolution and chemiluminescence intensity, a coupled column ch...
متن کاملEthyl 2-(3-amino-4-hydroxyphenyl)acetate
The asymmetric unit of the title compound, C(10)H(13)NO(3), contains two crystallographically independent mol-ecules with different conformations of the eth-oxy-carbonyl groups; the terminal C-C-O-C torsion angles in the two mol-ecules are 83.6 (6) and -171.1 (3)°, resulting in twisted and straight chain conformations, respectively. The crystal structure is stabilized by inter-molecular N-H⋯O, ...
متن کاملChemiluminescence Properties of Peroxyoxalate System in Organic Solvents
Peroxyoxalate system includes an oxalate ester, peroxide and a fluorescer, The energy resulted from the reaction of oxalate ester and peroxide is transferred to fluorescer and cause it to fluorsc. The effect of reactants chemical structure, their concentration, solvent media (polarity and viscosity) and the temperature, on the intensity of the illuminated light with respect to time have bee...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: American Journal of Analytical Chemistry
سال: 2020
ISSN: 2156-8251,2156-8278
DOI: 10.4236/ajac.2020.111002